Autofluorescence generation and elimination: a lesson from glutaraldehyde.

Elimination of autofluorescence in immunofluorescence microscopy with digital image processing.

Autofluorescence can be a very disturbing factor in immunofluorescence microscopy. We present here a method to eliminate autofluorescence. The method is based on the fact that most autofluorescent compounds have broad-banded excitation and emission spectra, whereas specific fluorescent probes have narrow spectra. Two images are recorded and digitized, one at a wavelength exciting both the fluor...

Diagnosis of Keratitis by Cultivation, Fluorescence Microscopy and PCR has Uncovered that Acanthamoeba Emits Auto Fluorescence

The amphizoic protozoa Acanthamoeba species are able to be free-living amoebae in environment or as parasites in humans and animals. These species comprise nearly 30 species and they have an increased role as human pathogens causing encephalitis in the nervous system or keratitis in the eyes. In diagnosis of a clinical sample of eye contact lens, light microscopy showed a heavy growth of amoebo...

Self-assembled polyelectrolyte nanoparticles as fluorophore-free contrast agents for multicolor optical imaging.

In this work, we describe the fabrication of self-assembled polyelectrolyte nanoparticles that provide a multicolor optical imaging modality. Poly(γ-glutamic acid)(γ-PGA) formed self-assembled nanoparticles through electrostatic interactions with two different cationic polymers: poly(L-lysine)(PLL) and chitosan. The self-assembled γ-PGA/PLL and γ-PGA/chitosan nanoparticles were crosslinked by g...

Elimination of Autofluorescence in Immunofluorescence

The Important Role of Lesson Plan on Educational Achievement of Iranian EFL Teachers' Attitudes

Lesson plan is a written description of education process in which it is shown what, when, where and with which method learners should learn and how they should be assessed. Lesson plan is one of the key factors in the educational process. According to the literature available, unfortunately few studies have been conducted on this issue in the context of Iran. Therefo...